Evaluation of disposable filtration systems for harvesting high cell density fed batch processes

Evaluation of disposable filtration systems for harvesting high cell density fed batch processes

Introduction In the underlying study we evaluated different single-use filtration systems for cell separation and harvest clarification in 1,000 L scale. A screening of different depth filters was carried out with various single-use filters from Pall, Cuno (3M), Millipore and Sartorius Stedim. In total, we included 85 depth filtrations in the screening. Out of that, two single-use filtration sy...

Batch and High Cell Density Fed-batch Culture Productions of an Organophosphorus Hydrolase

Organophosphorus hydrolases (OPH) are of great interest to the U. S. Army for their potential use in the non-toxic, non-corrosive decontamination against VX. OPH genes had been cloned and over expressed in Escherichia coli by other researchers. However, a common problem has been that of a low expression level. In this study, batch cultures in both complex (e.g., Lauria Broth) and minimal (succi...

media and specific growth rate selection for high-cell-density cultivation of recombinant escherichia coli producing hGM-CSF in fed-batch process

Intelligent Software Sensors for Fed-Batch Fermentation Processes

Software sensors have attracted great research interests due to the problem of lacking suitable and robust online sensors for key fermentation variables in fed-batch fermentation processes. In this paper, intelligent software sensors have been developed based on multivariate statistical process control methods. The software sensors not only provide real time estimation of key variables but also...

High Cell Density Fermentation of Saccharomyces cerevisiae JUL3 in Fed-batch Culture for the Production of β-Glucan

β-Glucan is a cell wall component that is one of the most plentiful cell polysaccharides. Moreover, it has been found to have several beneficial effects on the immune system. In yeast, β-glucan is mainly contained in the yeast cell wall, and thus it is important to produce high levels of cell mass for the mass production of yeast β-glucan. Response surface methodology (RSM) offers a potential m...